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  • 10969 Interleukin-6 Induced Acute Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity 2017-2-6
    Interleukin-6 Induced “Acute” Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics

  • 7554 Comprehensive identification of novel proteins and N-glycosylation sites 2017-2-4
    Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2†, Bin Han1†, Rongli Li1, Xiaoshan Lu1,3, Aiying Nie4, Lihai Guo5, Yu

  • 4322 Comparative assessment of glycosylation of recombinant human FSH and highly purified FSH 2017-1-22
    Comparative assessment of glycosylation of a recombinant human FSH and a highly purified FSH extracted from human urineHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui

  • 7632 KERRY細(xì)胞營養(yǎng)產(chǎn)品在制備無血清/低血清培養(yǎng)基中的應(yīng)用 2016-11-4
    基礎(chǔ)培養(yǎng)基+超濾植物源營養(yǎng)物=個性化低成本無血清/低血清培養(yǎng)基如此簡單高效!CHO細(xì)胞專用復(fù)合添加物Sheff-CHO系列專為CHO細(xì)胞優(yōu)化研發(fā);全球獨(dú)家非動物源復(fù)合添加物系統(tǒng);獨(dú)家超濾系統(tǒng)過濾;有

  • 2947 魚胰蛋白酶(trypsin)ELISA試劑盒操作步驟 2016-9-26
    我司ELISA試劑盒品質(zhì)保證,質(zhì)量優(yōu),價格實(shí)惠,是您生物實(shí)驗(yàn)的首選,如有需要可與我司銷售人員聯(lián)系。本試劑僅供研究使用目的:本試劑盒用于測定魚小腸內(nèi)胰蛋白酶 (trypsin)的活性。實(shí)驗(yàn)原理:本試

  • 5419 幾種蛋白質(zhì)濃度測定方法介紹 2016-6-13
    一、Bradford法蛋白質(zhì)與考馬斯亮蘭染料結(jié)合生成藍(lán)色物質(zhì),藍(lán)色物質(zhì)的量與蛋白質(zhì)濃度的高低成正比。生成的藍(lán)色物質(zhì)在595nm處有最大光吸收,通過測定595nm的光吸收,來測定蛋白質(zhì)濃度。實(shí)驗(yàn)中一般

  • 5086 BioTNT樣品處理之不同品牌糖尿病檢測試劑盒樣品處理對比說明 2016-1-20
    品牌:Shibayagi產(chǎn)品:Insulin ELISA Kit, Leptin ELISA Kit樣品準(zhǔn)備:1. 樣品最好現(xiàn)提現(xiàn)用,或-35度保存幾天之內(nèi),解凍的樣品需充分混勻后使用。2. 溶血和血脂含量過高的樣品不宜使用。若需長

  • 8101 通過反硝化細(xì)菌和激光同位素分析儀測定溶解硝酸鹽中的δ15N和δ18O 2015-7-21
    LGR氧化亞氮同位素分析儀測量水中硝酸鹽成功案例:Analytical Chemistry 文章:Combining Denitrifying Bacteria and Laser Spectroscopy for Isotopic Analyses (δ15N, δ18O) of Dissolved N

  • 6306 人全血Foxp3實(shí)驗(yàn)步驟 2014-12-3
    需要試劑 HumanRegulatoryTCellWholeBloodStainingKit(#88-8996-40) 包含組分: (1)、1XRBCLysisBuffer:200mL (2)、FlowCytometryStainingBuffer:600mL (3)、Fixation/PermeabilizationCon

  • 2511 mCherry標(biāo)簽抗體—可進(jìn)行WB/IF應(yīng)用的mCherry單抗 2013-9-13
    mCherry是一種被廣泛用于生物技術(shù)作為示蹤劑的紅色熒光染料,包括分子的標(biāo)記和細(xì)胞組分的定位等。不同于其它從維多利亞水母中分離的GFP蛋白和其它綠色熒光蛋白變體,mCherry以及其它大多數(shù)紅色熒

  • 3803 CompresstomeTM應(yīng)用II 基因功能研究:組織學(xué)連續(xù)切片 2013-6-21
    儀器應(yīng)用手冊Volume 7 CompresstomeTM應(yīng)用II 基因功能研究:組織學(xué)連續(xù)切片關(guān)鍵詞:組織學(xué)(Histology), 基因功能(Gene Function), 免疫組織化學(xué)(Immunohistochemistry), 連續(xù)切片(Serial

  • 2830 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
    Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The

  • 1802 Isolation of papillary cells 2012-3-26
    Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept

  • 3912 Isolation of human primary ovarian surface epithelial cells 2011-12-12
    Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut

  • 2294 Primary brain cell isolation and culture 2011-6-13
    Primary brain cell isolation and culture. 1.Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containin

  • 2324 Measurement of primary endothelial cell permeability to fluxes of dextran or albumin 2011-4-1
    Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell (ECs) were evaluated using Tra

  • 2395 Ex Vivo Human Primary Mesenchymal Stem Cells (MSCs) Culture
 2011-4-1
    Ex Vivo Human Primary Mesenchymal Stem Cells (MSCs) Culture Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs). MSCs produce adventitial cells in th

  • 4046 海螺毒素多肽的特性及其應(yīng)用 2010-6-30
    海螺產(chǎn)生的毒素(conotoxins)按結(jié)構(gòu)不同可作用于鈉或鉀離子通道上的受體,其獨(dú)特的性能使它們成為各研究領(lǐng)域里有價值的工具。 地理錐芋螺 僧枹芋螺 光環(huán)芋螺來自獵魚海螺地理錐芋螺毒液中的

  • 5811 蛋白定量--Lowry法[wkh] 2008-12-26
    蛋白定量--Lowry法[wkh] 2008-07-28 20:15:35Folin—酚試劑法(Lowry法)(一)實(shí)驗(yàn)原理這種蛋白質(zhì)測定法是最靈敏的方法之一。過去此法是應(yīng)用最廣泛的一種方法,由于其試劑乙的配制較為困難(

  • 5506 流式細(xì)胞儀原理及及其在植物上的應(yīng)用和選用 2008-3-22
    摘要:隨著科技水平的不斷深入,科研設(shè)備的不斷更新,國家對科研投入的不斷增多,植物檢測技術(shù)也得到了進(jìn)一步的發(fā)展。流式細(xì)胞儀植物檢測技術(shù)是近幾年在我國剛剛興起的一項(xiàng)新技術(shù),對許多植物科

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