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SDF 1a Human

英文名稱：	SDF 1a Human	總訪問：	269
國產/進口：	進口	半年訪問：	1
產地/品牌：	prospec	產品類別：	細胞生物學試劑
規(guī) 格：	CHM-262	最后更新：	2024-12-5
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公司簡介

Catalogue Number

CHM-262

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.

Introduction

SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow).
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive (unlike for other chemokines and their receptors), but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.

Description

Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8004 Dalton.
The SDF-1a is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.

Protein content

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of SDF-1a as a Reference Standard.

References

Title: Stromal Cell-Derived Factor-1a Promotes Neuroprotection, Angiogenesis, and Mobilization/Homing of Bone Marrow- Derived Cells in Stroke Rats.

Publication: THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Copyright © 2008 by The American Society for Pharmacology and Experimental Therapeutics JPET 324:834–849, 2008. Vol. 324, No. 2 127746/3301181 Printed in U.S.A.

Link: http://jpet.aspetjournals.org/content/324/2/834.full.pdf

Application: Intracerebral administration of SDF-1 resulted in neuroprotection against neurotoxic insult, and it induced increased BM-derived cell targeting to the ischemic brain, thereby reducing the volume of cerebral infarction and improving neural plasticity.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not>

北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領域多年銷售經驗人士創(chuàng)立。目前我公司已經與幾十家著名的試劑供應商建立了長期穩(wěn)定的合作關系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內客戶的最新需求，我們也與時俱進地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標是把國外有特點的生物前沿產品引進中國，讓國內科學研究機構和制藥企業(yè)盡早使用上最新型的科研產品。

我們代理的產品覆蓋細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發(fā)生產等領域。

細胞生物學包含細胞培養(yǎng)，細胞遷移，細胞信號轉導等。

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