ADRA1A人腎上腺素能a1A受體檢測試劑盒

ADRA1A人腎上腺素能a1A受體檢測試劑盒雙抗體夾心法檢測抗原，間接法檢測抗體，為客戶提供各種ELISA技術進行樣本檢測。

英文縮寫：ADRA1A Elisa kit

產品關鍵詞：腎上腺素能a1A受體酶聯(lián)免疫試劑盒

規(guī)格96T/48T

保存條件：2-8℃低溫保存

運輸：快遞運輸（可根據(jù)客戶要求，選擇具體的運輸方式，無特殊要求我公司一般為順豐）。

ADRA1A人腎上腺素能a1A受體檢測試劑盒樣品準備:

1. Acidify the plasma/serum with an equal amount of buffer A. For example, if you are using 1ml of plasma/serum, add 1 ml of buffer A. Mix and centrifuge at 6,000 to 17,000 x g for 20 minutes at 4°C.

2. Equilibrate a SEP-COLUMN containing 200 mg of C18 (Catalog No.: RK-SEPCOL-1) by washing with buffer B (1 ml, once) followed by buffer A (3 ml, 3 times).NOTE: From steps 3-5, no pressure should be applied to the column.

3. Load the acidified plasma/serum solution onto the pre-treated (Step 2) C-18 SEP-COLUMN.

4. Slowly wash the column with buffer A (3 ml, twice) and discard the wash.

5. ADRA1A人腎上腺素能a1A受體檢測試劑盒Elute the peptide slowly with buffer B (3 ml, once) and collect eluant into a polystyrene tube.

6. Evaporate eluant to dryness in a centrifugal concentrator or by a suitable substitute method.

7(i). For EIA kits, Fluorescent EIA kits and Chemiluminescent EIA kits, keep the dried extract at -20°C and perform the assay as soon as possible. For normal subject extracted from 1 ml original plasma/serum, use 125 ul 1x assay buffer to reconstitute the dried extract. Aliquot 50 ul into two designated assay wells (25 ul is left over). the concentration factor in this case is 8. (1 ml / 125 ul = 8). The original plasma peptide level is 1/8 of the level of final extracted plasma.

ADRA1A人腎上腺素能a1A受體檢測試劑盒樣本要求:

在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集標本，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質量，所以避免反復凍融。代測放免標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術人員溝通。

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