ROCK-1 (cleaved)抗體

【背景資料】：ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.

【應用類型】：Western blotting (1-4 μg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate treated with camptothecin (2 μM for 6-8 hours) can be used as a positive control. The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.

小鼠

【反應性】：經(jīng)檢測驗證，可與人、小鼠種屬來源的ROCK-1 (cleaved)蛋白 發(fā)生免疫反應。

【保存建議】：提供的ROCK-1 (cleaved)抗體 產(chǎn)品為100 μg 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)緩沖液中。請將該ROCK-1 (cleaved)抗體 產(chǎn)品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融。

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