高表達(dá)vimentin的巨噬細(xì)胞在肝細(xì)胞癌免疫抑制中的關(guān)鍵作用

Vimentin (波形蛋白) 是一種在間充質(zhì)細(xì)胞中廣泛表達(dá)的 III 型中間絲 (intermediate filament, IF) 蛋白。它在細(xì)胞骨架的形成、細(xì)胞的形狀維持、細(xì)胞的遷移、細(xì)胞信號傳導(dǎo)以及細(xì)胞應(yīng)激反應(yīng)中發(fā)揮著重要作用。它通過側(cè)向或末端方式附著于細(xì)胞核、內(nèi)質(zhì)網(wǎng)和線粒體，參與調(diào)控細(xì)胞定向運動、取向排列、細(xì)胞片層組織形成，并在細(xì)胞遷移前沿維持高爾基體極性。此外，波形蛋白通過細(xì)胞接觸依賴機制保護(hù)SCRIB蛋白免受蛋白酶體降解，并促進(jìn)其定位于中間絲結(jié)構(gòu)

Vimentin結(jié)構(gòu). Schematic representation of a vimentin filament from a monomer to its assembly into a mature filament. The vimentin monomer is composed of a central a-helical domain flanked by an unstructured head and tail domain. On the central domain, vimentin possesses a rod II domain with lectin-like properties.

重要發(fā)現(xiàn)：Vimentin高表達(dá)（VIM+）巨噬細(xì)胞通過分泌IL-1β激活Treg細(xì)胞的免疫抑制功能

復(fù)旦大學(xué)的王紅陽/陳磊教授團(tuán)隊于2024年9月在Nature Cancer （IF：23.5）上發(fā)表了題為“Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma”的研究性文章。該文繪制了HCC單細(xì)胞空間蛋白圖譜，解析了HCC腫瘤間空間異質(zhì)性，建立了預(yù)后相關(guān)的干細(xì)胞癌（HCC）空間分型（Spatial Pattern），并整合多組學(xué)和體外實驗揭示了Vimentin高表達(dá)的巨噬細(xì)胞與Treg細(xì)胞的空間互作促進(jìn)HCC的疾病進(jìn)展。

該項研究通過開展了單細(xì)胞測序和空間轉(zhuǎn)錄組分析，揭示了VIM+巨噬細(xì)胞的基因表達(dá)特征，發(fā)現(xiàn)了VIM+巨噬細(xì)胞和Treg之間基因表達(dá)的高度關(guān)聯(lián)性，提示了VIM+巨噬細(xì)胞可能作用于Treg，從而增強免疫抑制。配受體分析進(jìn)一步揭示了VIM+巨噬細(xì)胞或許通過白介素-1β（IL-1β）和干擾素-γ（IFN-γ）參與對Treg細(xì)胞的調(diào)控。隨后，作者利用流式細(xì)胞儀分離了VIM+和VIM低表達(dá)的巨噬細(xì)胞，并檢測了其中IL-1β的表達(dá)水平。結(jié)果表明，在不同類型的巨噬細(xì)胞中，VIM+的巨噬細(xì)胞顯著高表達(dá)IL-1β，細(xì)胞共培養(yǎng)實驗發(fā)現(xiàn)了VIM+巨噬細(xì)胞促進(jìn)Treg分泌IL-10，而加入IL-1β抗體可顯著緩解作用效果。

斯達(dá)特S-RMab® 
Vimentin Recombinant Rabbit mAb (SDT-029-120) （貨號：S0B2254）抗體是您Vimentin 研究中的有力幫手。

數(shù)據(jù)驗證：
WB免疫印記

WB result of Vimentin Rabbit mAb
Primary antibody: Vimentin Rabbit mAb at 1/500 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 57 kDa

IHC 免疫組化

斯達(dá)特S-RMab® 系列抗體賦能生命科學(xué)和病理研究
免疫細(xì)胞化學(xué)

ICC shows positive staining in HeLa cells. Anti-Vimentin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).

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杭州斯達(dá)特 (www.starter-bio.com)志在為全球生命科學(xué)行業(yè)提供優(yōu)質(zhì)的抗體、蛋白、試劑盒等產(chǎn)品及研發(fā)服務(wù)。依托多個開發(fā)平臺：重組兔單抗、重組鼠單抗、快速鼠單抗、重組蛋白開發(fā)平臺（E.coli,CHO,HEK293,InsectCells）,已正式通過歐盟98/79/EC認(rèn)證、ISO9001認(rèn)證、ISO13485。