DNA-蛋白質(zhì)互作研究方法

Cleavage Under Target & Tagmentation (CUT&Tag)是一種新興的DNA-蛋白質(zhì)互作研究方法。CUT&Tag以融合了protein A/G的Tn5轉(zhuǎn)座酶為核心，融合蛋白通過protein A/G與抗體結合，使得Tn5被栓系在靶位點周圍，從而在目的位點附近進行酶切反應，并同時引入二代測序所必需的接頭序列，產(chǎn)物DNA經(jīng)過后續(xù)提取與PCR擴增后，得到直接用于測序的文庫。CUT&Tag與傳統(tǒng)的ChIP-Seq技術相比較，實驗不需要超聲打斷，也不需要傳統(tǒng)的連接法添加測序接頭，操作簡單、周期短、信噪比高、重復性好、細胞用量少，為極低起始細胞量和單細胞測序研究提供了可能性。

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